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Technology
Analysis that looks for differences in the lengths of microsatellite repeats between alleles. Polymorphic microsatellites are present in the genome every few thousand base pairs and can function as molecular markers.
Technology
Emission of electromagnetic energy with a shorter frequency (longer wavelength) than that of the incident monochromatic light. Arises from the low probability absorption of quanta with a higher energy than that required for a transition: the difference in energy is emitted as a lower frequency (energy) photon. Allows analysis of vibrational and rotational energy levels using visible incident light.
Technology
A procedure used to demonstrate nucleotide sequence variations that occur in the same region of DNA between individuals within a species, or between alleles in an individual to detect mutations in various genes, such as oncogenes, tumor suppressor genes, or genes responsible for genetic diseases. The method detects different conformations adopted by partially denatured DNA fragments resulting from their specific nucleotide sequences under denaturing conditions. Denatured single-stranded DNA fragments are allowed to partially renature without formation of double-stranded DNA. The particular conformations adopted by each of the renatured fragments depend on each of their specific nucleotide sequences. The fragments are run on polyacrylamide gels to detect subtle changes in migration due to their particular secondary structures. Resulting fragment band in the gel will misalign if any point mutations are present to alter the conformation.
Technology
Mass spectrometers coupled in series. The targeted compound is selectively ionized, and its characteristic ions are separated from others in the mixture in the first mass spectrometer. The selected primary ions are then decomposed by collision, and from the resulting products the final mass analyzer selects secondary ions characteristic of the targeted compound. Tandem mass spectrometry can achieve specificities and sensitivities equivalent of those of methods such as radioimmunoassay and gas chromatography/mass spectrometry, while performing analyses in much shorter times.
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Article
Research record from Zhonghua Zhong Liu Za Zhi, 2026-07-01. PMID 42477931.
Article
Research record from Se Pu, 2026-03-01. PMID 41814904.
Article
Research record from Zhonghua Er Ke Za Zhi, 2026-03-14. PMID 41834204.
