Glossary Term
Read Length
The number of nucleotides successfully ordered from each side of a nucleic acid fragment obtained after the completion of a sequencing process.
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Glossary Term
The number of nucleotides successfully ordered from each side of a nucleic acid fragment obtained after the completion of a sequencing process.
Glossary Term
One of the three possible ways of reading a nucleotide sequence. As the genetic code is read in nonoverlapping triplets (codons) there are three possible ways of translating a sequence of nucleotides into a protein, each with a different starting point.
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An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
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A PCR method that measures amplification as it happens using fluorescent reporters, so target quantity is estimated from the amplification curve rather than by post-run handling. qPCR is the workhorse format for viral load, pathogen detection, and GMO or food testing workflows.
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Complementary precision measures: repeatability is variation under the same conditions (same operator, equipment, short interval); reproducibility is variation across conditions — different days, operators, lots, or laboratories.
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The ability to yield similar results given a set of constraints.
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A study in which participants with known outcomes are assessed based on data collected before the study.
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An RNA-dependent DNA polymerase that is involved in the replication of retroviruses an in the maintenance of telomeres.
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The action of copying RNA into DNA by reverse transcriptase.
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Ribonuclease inhibitor (461 aa, ~50 kDa) is encoded by the human RNH1 gene. This protein plays a role in the inhibition of ribonucleases.
Glossary Term
Single-stranded long chain of nucleotides containing ribose. It is the end product of DNA transcription by the enzyme RNA polymerase. It is essential in protein synthesis.
Glossary Term
An RNA molecule that does not contain protein-coding sequence information and constitutes the majority of the cellular RNA (~80%). This nucleic acid associates with multiple proteins to form ribosomes, which are the organelles that drive mRNA-directed protein synthesis.