National Cancer Institute (source definition)
Transcription-mediated Amplification
A technique used to selectively replicate nucleic acid sequences from RNA templates using reverse transcriptase and RNA polymerase. Reverse transcriptase generates cDNA from the region of interest using primers and then RNA polymerase generates copies of the original RNA template.
Review and references
Automated source check (NCI definition)
2026-10-18
2026-10-19
professional use
References
- NCI Thesaurus 26.09d — Transcription-mediated Amplification (C122175)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C122175. NCI definition, reproduced without modification under CC BY 4.0.
- Comparison of diagnostic performance of transcription-mediated amplification with chemiluminescent microparticle immunoassay in the detection of hepatitis C virus in dialysis patient group. — Asian J Transfus Sci, 2026-01-01 (PMID 42344660; literature)
- Heightened Nontesting Risk Mitigation Blood Donor Screening with Retrospective Transcription-Mediated Amplification Testing during a 2023 Autochthonous Florida Malaria Cluster. — Am J Trop Med Hyg, 2025-12-09 (PMID 41662735; literature)
What it is
A technique used to selectively replicate nucleic acid sequences from RNA templates using reverse transcriptase and RNA polymerase. Reverse transcriptase generates cDNA from the region of interest using primers and then RNA polymerase generates copies of the original RNA template.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C122175. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.