National Cancer Institute (source definition)
Southern Blotting
A technique for the detection of specific DNA fragments that have been separated by polyacrylamide-gel electrophoresis, transferred to a nitrocellulose or other type of paper or nylon membrane, and detected by hybridization and visualization with a labeled probe.
Review and references
Automated source check (NCI definition)
2026-10-18
2026-10-19
professional use
References
- NCI Thesaurus 26.09d — Southern Blotting (C16356)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C16356. NCI definition, reproduced without modification under CC BY 4.0.
- Optimized Southern blotting for enhanced and precise detection of transgenes in CHO cells from transposon-based expression systems. — Biotechniques, 2026-01-29 (PMID 41609232; literature)
- Southern Blotting: A Valuable Molecular Technique to Verify Gene Replacement in Ustilago maydis. — Methods Mol Biol, 2026-01-01 (PMID 42681038; literature)
What it is
A technique for the detection of specific DNA fragments that have been separated by polyacrylamide-gel electrophoresis, transferred to a nitrocellulose or other type of paper or nylon membrane, and detected by hybridization and visualization with a labeled probe.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C16356. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.