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Product

Universal Internal Control Template 1.0 (RUO)

Molecular Assays

Technology

Anchored Multiplex Polymerase Chain Reaction

A technique designed to rapidly enrich and amplify targets derived from samples that yield low levels of nucleic acids, such as formalin-fixed paraffin-embedded (FFPE) tissues, for use in next-generation sequencing. Briefly, total nucleic acid or genomic DNA (gDNA) is isolated from fresh or FFPE tissues. Total nucleic acid is converted to double-stranded cDNA (dscDNA) and cleaned by solid-phase reversible immobilization (SPRI). Then the dscDNA or gDNA is subjected to end repair, adenylation and ligation with a universal half-functional adapter. The modified DNA sample is then subjected to two rounds of nested low-cycle PCR, where the first round uses target gene-specific primers and the second round uses nested target gene-specific primers that are 5' tagged with a common sequencing adapter. The resulting target amplicon library is cleaned using SPRI and is suitable for use in next generation genomic assays.

Diagnostic Methods

Product

Trueprep AUTO v2 Universal Cartridge Based Sample Prep Kit

Trueprep AUTO v2 Universal Cartridge Based Sample Prep Kit is catalogued from public product materials published by Molbio Diagnostics Pvt. Ltd. Availability: not published on this record. Regulatory status is manufacturer-reported and has not been independently verified. Confirm intended use, compatibility and local registration against official documentation before procurement.

Molecular Assays

Product