Technology
Multiplex PCR Guide: Panel Design, Use Cases & Performance Trade-offs
Learn how multiplex PCR balances panel breadth, sensitivity, cross-reactivity and instrument compatibility across clinical applications.
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Technology
Learn how multiplex PCR balances panel breadth, sensitivity, cross-reactivity and instrument compatibility across clinical applications.
Technology
Polymerase chain reaction that uses two sets of primers, in two sequential runs of amplification, with the second set intended to amplify a target within the first run product.
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Compare magnetic bead, column-based, manual and automated extraction methods for different sample types and downstream PCR workflows.
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Estimate workflow fit by sample volume, turnaround expectations, staffing and automation tier for PCR labs.
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Compare automation levels for PCR workflows by hands-on time, throughput, contamination risk and staffing requirements.
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A method for amplifying a DNA base sequence using multiple rounds of heat denaturation of the DNA and annealing of oligonucleotide primers complementary to flanking regions in the presence of a heat-stable polymerase. This results in duplication of the targeted DNA region. Newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, so that successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.
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Quantitative PCR: a method to measure very small amounts of RNA transcripts as cDNA targets. There are two strategies currently used, one is called Competitive RTPCR and the other is called Real-Time PCR.
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An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
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A laboratory procedure in which an RNA strand is first transcribed into a DNA complement and then subjected to PCR amplification. Transcribing an RNA strand into a DNA complement is termed reverse transcription and is done by the enzyme reverse transcriptase.
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Match sample types with extraction, assay and workflow considerations for practical PCR planning.
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Plan PCR throughput by daily sample volume, turnaround targets, staffing model and automation fit across low to high volume labs.