Glossary Term
Dideoxy Chain Termination DNA Sequencing
PCR TermsEN
Definition
A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs, which are radioactively or fluorescently labeled, are combined within the reaction mixture. Once the reaction is complete, the DNA strands are separated by size, and the labeled chain terminating dNTPs can be read in sequence by the investigator or by a machine.
Extended Notes
Source: National Cancer Institute, NCI Thesaurus 26.09d, C19641. NCI definition, reproduced without modification under CC BY 4.0. Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Also Known As
Dideoxy Chain Termination DNA SequencingSanger Sequencing