Technology
LoD, Precision and PCR Performance Guide for Validation & Procurement
Evaluate limit of detection, precision, repeatability and evidence strength for PCR product validation, tenders and procurement review.
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Technology
Evaluate limit of detection, precision, repeatability and evidence strength for PCR product validation, tenders and procurement review.
Technology
Check PCR instrument compatibility by fluorescence channels, reaction format support, software fit and validation effort.
Technology
Check assay-to-instrument fit by fluorescence channels, reaction format and software compatibility across installed PCR platforms.
Technology
Variation occurring within a species in the length of DNA fragments generated by a specific endonuclease. Such variations are generated by mutations that create or abolish recognition sites for these enzymes.
Technology
A procedure used to demonstrate nucleotide sequence variations that occur in the same region of DNA between individuals within a species, or between alleles in an individual to detect mutations in various genes, such as oncogenes, tumor suppressor genes, or genes responsible for genetic diseases. The method detects different conformations adopted by partially denatured DNA fragments resulting from their specific nucleotide sequences under denaturing conditions. Denatured single-stranded DNA fragments are allowed to partially renature without formation of double-stranded DNA. The particular conformations adopted by each of the renatured fragments depend on each of their specific nucleotide sequences. The fragments are run on polyacrylamide gels to detect subtle changes in migration due to their particular secondary structures. Resulting fragment band in the gel will misalign if any point mutations are present to alter the conformation.