Glossary Term
Read Depth
The number of times a particular locus (site, nucleotide, amplicon, region) was sequenced.
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Glossary Term
The number of times a particular locus (site, nucleotide, amplicon, region) was sequenced.
Glossary Term
A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs, which are radioactively or fluorescently labeled, are combined within the reaction mixture. Once the reaction is complete, the DNA strands are separated by size, and the labeled chain terminating dNTPs can be read in sequence by the investigator or by a machine.
Glossary Term
The number of nucleotides successfully ordered from each side of a nucleic acid fragment obtained after the completion of a sequencing process.
Glossary Term
One of the three possible ways of reading a nucleotide sequence. As the genetic code is read in nonoverlapping triplets (codons) there are three possible ways of translating a sequence of nucleotides into a protein, each with a different starting point.