Technology
This is a method (named after the Danish bacteriologist, Hans Christian Gram) for staining and identifying bacteria. It is based on the differential retention of a crystal violet-iodine complex within the cell membrane. Gram-positive organisms will appear blue-black or purple while Gram-negative organisms will appear red.
Diagnostic Methods
Technology
A type of chromatography assay in which an antibody-conjugate is passed over an immobilized antigen, giving a visual indication of positive or negative result.
Diagnostic Methods
Technology
Understand internal, positive, negative and external controls for reliable PCR testing, inhibition monitoring and audit-ready QC workflows.
Internal Control Qc
Technology
Analytical and separative procedures in which molecules, particularly proteins or nucleic acids, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. For proteins, commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight.
Diagnostic Methods