Glossary Term
Contaminated
The presence of any substance or organism that makes a preparation impure.
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Glossary Term
The presence of any substance or organism that makes a preparation impure.
Glossary Term
Enzymatic (e.g. dUTP/UNG) and physical measures that stop amplified DNA from contaminating later runs. Labs with high daily volumes usually require dUTP/UNG chemistry in purchased assays; confirm this when comparing kits from different manufacturers.
Glossary Term
A reaction that contains all reagents except sample nucleic acid. Amplification in the NTC signals contamination of reagents or the environment, so NTC behavior is one of the first run-validity checks in any qPCR run log.
Glossary Term
Any of various amplification and detection strategies applied to detection of virus contamination in donor blood. Specifically designed to detect recently infected individuals during the latency period before seroconversion.