Technology
Internal Control and QC for PCR Testing: Reliability, Inhibition & Documentation
Understand internal, positive, negative and external controls for reliable PCR testing, inhibition monitoring and audit-ready QC workflows.
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Technology
Understand internal, positive, negative and external controls for reliable PCR testing, inhibition monitoring and audit-ready QC workflows.
Technology
An assay performed to assess platelet clumping, often with the addition of agonists.
Technology
A method for amplifying a DNA base sequence using multiple rounds of heat denaturation of the DNA and annealing of oligonucleotide primers complementary to flanking regions in the presence of a heat-stable polymerase. This results in duplication of the targeted DNA region. Newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, so that successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.
Technology
A DNA sequencing method that uses a "sequencing by synthesis" strategy. The strand complementary to a template is synthesized a single base at a time through the sequential addition of individual solutions of each base. Incorporation of a base into the growing strand generates free pyrophosphate that is detected as light, as generated by a luciferase-catalyzed reaction.