Technology
A system of (usually) epifluorescence light microscopy in which a fine laser beam of light is scanned over the object through the objective lens. The technique is particularly good at rejecting light from outside the plane of focus and so produces higher effective resolution than is normally achieved.
Diagnostic Methods
Technology
Electron microscopy involving an aqueous suspension of a sample which is applied in an extremely thin film to a grid, frozen in liquid nitrogen and maintained in this state by means of a special mount. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.
Diagnostic Methods
Technology
Branch of histochemistry associated with the localization of cellular components by specific staining methods, as for example the localization of acid phosphatases by the Gomori method.
Diagnostic Methods
Technology
Techniques for analysis of chromosomal and subchromosomal properties and structures, such as those to diagnose, classify, screen for, or manage genetic diseases and abnormalities.
Diagnostic Methods
Technology
The light microscopic study of normal and abnormal cells in fine needle aspirates (FNAs), body cavity fluids, and smears.
Diagnostic Methods
Technology
A light microscopic technique in which the light that does not come into contact with the structures or details of interest is subtracted from the ocular image. This yields an image in which the structures or details are alight while the areas where the structures or details are absent are dark.
Diagnostic Methods
Technology
A method of separating macromolecules by either their differential rate of sedimentation in a centrifugal gradient or their differential buoyancy in a density gradient. (from biology-text.com)
Diagnostic Methods
Technology
A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs, which are radioactively or fluorescently labeled, are combined within the reaction mixture. Once the reaction is complete, the DNA strands are separated by size, and the labeled chain terminating dNTPs can be read in sequence by the investigator or by a machine.
Diagnostic Methods
Technology
A type of polymerase chain reaction technique in which the sample is fractionated, within which individual PCR reactions occur in each fraction.
Diagnostic Methods
Technology
A measurement of the antibody or complement-coated erythrocytes in a blood specimen in vivo.
Diagnostic Methods
Technology
Identification of an individual's genetic information from their DNA.
Diagnostic Methods
Technology
Any method that extracts and purifies DNA from biological samples, which can include the proper handling of biological samples prior to nucleic acid isolation, the separation of DNA from the rest of the components in the samples, and the storage of the DNA isolates.
Diagnostic Methods