Glossary Term
A reaction that contains all reagents except sample nucleic acid. Amplification in the NTC signals contamination of reagents or the environment, so NTC behavior is one of the first run-validity checks in any qPCR run log.
Pcr Terms
Glossary Term
The ratio of the odds of an event occurring in one group to the odds of it occurring in another group, or to a sample-based estimate of that ratio.
Performance Metrics
Glossary Term
Any of the 99 numbered points that divide an ordered set of scores into 100 parts each of which contains one-hundredth of the total.
Performance Metrics
Glossary Term
Quantity of dimension one used to express on a scale from 0 to 14 the amount-of-substance concentration of hydrogen ion of dilute aqueous solution, calculated as the logarithm of the reciprocal of hydrogen-ion concentration in gram atoms per liter.
Pcr Terms
Glossary Term
One of the three possible ways of reading a nucleotide sequence. As the genetic code is read in nonoverlapping triplets (codons) there are three possible ways of translating a sequence of nucleotides into a protein, each with a different starting point.
Pcr Terms
Glossary Term
Impregnation of one substance by another to the greatest possible extent; that concentration of a dissolved substance that cannot be exceeded.
Performance Metrics
Glossary Term
The act or process of rendering from one language into another.
Pcr Terms
Glossary Term
The movement of a physical object from one place to another. (NCI)
Pcr Terms
Glossary Term
A statistical hypothesis test that controls directional errors if rejection of a hypothesis is the result of a directional decision. The two-sided test has less statistical power than the one-sided test.
Performance Metrics