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Technology
Emission of electromagnetic energy with a shorter frequency (longer wavelength) than that of the incident monochromatic light. Arises from the low probability absorption of quanta with a higher energy than that required for a transition: the difference in energy is emitted as a lower frequency (energy) photon. Allows analysis of vibrational and rotational energy levels using visible incident light.
Technology
A procedure used to demonstrate nucleotide sequence variations that occur in the same region of DNA between individuals within a species, or between alleles in an individual to detect mutations in various genes, such as oncogenes, tumor suppressor genes, or genes responsible for genetic diseases. The method detects different conformations adopted by partially denatured DNA fragments resulting from their specific nucleotide sequences under denaturing conditions. Denatured single-stranded DNA fragments are allowed to partially renature without formation of double-stranded DNA. The particular conformations adopted by each of the renatured fragments depend on each of their specific nucleotide sequences. The fragments are run on polyacrylamide gels to detect subtle changes in migration due to their particular secondary structures. Resulting fragment band in the gel will misalign if any point mutations are present to alter the conformation.
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Article
Research record from Zhonghua Zhong Liu Za Zhi, 2026-07-01. PMID 42477931.
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Research record from Se Pu, 2026-03-01. PMID 41814904.
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Research record from Zhonghua Er Ke Za Zhi, 2026-03-14. PMID 41834204.
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Research record from Zhongguo Shi Yan Xue Ye Xue Za Zhi, 2026-06-01. PMID 42544655.
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Research record from Se Pu, 2026-03-01. PMID 41814896.
