Glossary Term
Real Time PCR
An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
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Glossary Term
An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
Glossary Term
A PCR method that measures amplification as it happens using fluorescent reporters, so target quantity is estimated from the amplification curve rather than by post-run handling. qPCR is the workhorse format for viral load, pathogen detection, and GMO or food testing workflows.
Glossary Term
A two-step setup in which RNA is first copied into complementary DNA by a reverse transcriptase and then amplified by PCR. Used for RNA viruses such as influenza, RSV, SARS-CoV-2, and HCV. In procurement documents, confirm whether "RT-PCR" refers to real-time (quantitative) or endpoint (qualitative) reading.