Technology
A method for amplifying a DNA base sequence using multiple rounds of heat denaturation of the DNA and annealing of oligonucleotide primers complementary to flanking regions in the presence of a heat-stable polymerase. This results in duplication of the targeted DNA region. Newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, so that successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.
Diagnostic Methods
Technology
Analysis that looks for differences in the lengths of microsatellite repeats between alleles. Polymorphic microsatellites are present in the genome every few thousand base pairs and can function as molecular markers.
Diagnostic Methods
Technology
An electroanalytical technique in which the electrical potential of a solution is measured to determine the composition of the sample, by comparing the constant potential of the reference electrode with the actual potential of the indicator electrode.
Diagnostic Methods
Technology
The process of determining the sequence of amino acids within a protein molecule.
Diagnostic Methods
Technology
Pulsed-field gel electrophoresis (PFGE) is a method used to separate proteins or high molecular weight linear DNA fragments that are greater than 50 kilobases (kb) in size. Fragments are separated by pulsing an electrical field across an agarose gel rather than applying the constant field used in conventional electrophoresis.
Diagnostic Methods
Technology
A DNA sequencing method that uses a "sequencing by synthesis" strategy. The strand complementary to a template is synthesized a single base at a time through the sequential addition of individual solutions of each base. Incorporation of a base into the growing strand generates free pyrophosphate that is detected as light, as generated by a luciferase-catalyzed reaction.
Diagnostic Methods
Company
Combining the over 40-years experienced manufacturer of reagents, Cormay with the outstanding technology knowledge of Swiss hematology company, Orphee, we work together every day to
Manufacturer
Product
QIAstat-Dx Analyzer 2.0 is catalogued from public product materials published by QIAGEN N.V. Availability: not published on this record. Regulatory status is manufacturer-reported and has not been independently verified. Confirm intended use, compatibility and local registration against official documentation before procurement.
Molecular Assays
Product
QIAstat-Dx Automated Molecular Panel Tests is catalogued from public product materials published by QIAGEN N.V. Availability: not published on this record. Regulatory status is manufacturer-reported and has not been independently verified. Confirm intended use, compatibility and local registration against official documentation before procurement.
Molecular Assays
Technology
Quantitative PCR: a method to measure very small amounts of RNA transcripts as cDNA targets. There are two strategies currently used, one is called Competitive RTPCR and the other is called Real-Time PCR.
Diagnostic Methods
Product
QubeMDx+™ is catalogued from public product materials published by Credo Diagnostics. Availability: not published on this record. Regulatory status is manufacturer-reported and has not been independently verified. Confirm intended use, compatibility and local registration against official documentation before procurement.
Instruments
Technology
A quantitative technique used to detect and estimate the concentration of an antigen by measuring the diameter of the ring of precipitation (precipitin ring), which is formed as a result of the interaction of the antigen and an antibody at optimal concentration.
Diagnostic Methods
